Abstract

Research Article

The Healing and Aging-related Properties of Adipose Tissue Fragments Obtained through the Guided SEFFI Procedure’s Mechanical Fragmentation are Facilitated by the Exosomes Present in the Final Injection

Alessandro Casadei, Alessandro Gennai, Bruno Bovani, Tommaso Pusceddu, Lucia Sileo, Maria Pia Cavalieri, Martina Greco and Barbara Zavan*

Published: 01 May, 2024 | Volume 8 - Issue 1 | Pages: 010-015

The Injection of autologous Adipose-Derived Stem Cells (ADSCs) and Stromal Vascular Fraction (SVF) into dermal and subdermal layers can improve skin volume and rejuvenation. The SEFFI (Superficial Enhanced Fluid Fat Injection) technique, which involves minimal manipulation of autologous microfragmented adipose tissue, was utilized for harvesting and re-injection, using the SEFFILLER™ disposable medical device. Mechanical fragmentation of adipose tissue is a well-established surgical technique that stimulates tissue regeneration, filler, and biological activity. The study evaluated the biological properties (regenerative and anti-aging) of different harvest and processing fat graft methods among which the fragmented adipose tissue, specifically focusing on the presence of exosomes. Exosomes, nanometer-sized vesicles produced by cells for cellular communication, were found to contain miRNAs with anti-inflammatory, regenerative, and vascular content. The products’ contained exosomes were confirmed in the study through electron microscopy, Western Blotting, gene expression, and sequencing of miRNA content. 

Read Full Article HTML DOI: 10.29328/journal.jsctt.1001037 Cite this Article Read Full Article PDF

Keywords:

Fat grafting; Soft-tissue augmentation; Soft-tissue reconstruction; Regenerative medicine; ADSCs; Exosomes

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