Abstract

Research Article

Age-related changes in cell yield and viability of feline Adipose Tissue-Derived Mesenchymal Stem (fAD-MSCs)

Shazia Nissar*, Geetha Ramesh, Sabiha Hayath Basha, TA Kannan and Shahid Hussain

Published: 01 April, 2022 | Volume 6 - Issue 1 | Pages: 001-004

In the present study, omental adipose tissue was collected from, the animals that underwent ovariectomy and ovariohysterectomy, surgical procedures, at the age of seven months to 11/2 years of age groups. The sample was digested with 0.1% (W/V) collagenase type I and transferred to a beaker with a magnetic stirrer and kept in a stirrer with 600 rpm at 37 °C for 30 minutes. The viability of the cell was evaluated by the trypan blue exclusion method using a hemocytometer. Trypan blue had a high affinity to nuclear DNA, which traverse the member in a dead cell and dye it blue. In the present study, the cell yield of fAD-MSCs was 8.15 ± 0.68, 6.55 ± 0.26, 4.85 ± 0.42, 3.90 ± 0.34, and 3.51 ± 0.43 in different age groups viz., 7,8,9 month 1 and 1½ year respectively. In younger age groups, cell yield and viability percentage were more than in animals of higher age groups. In the younger age group, stem cells proliferation status is considered potent for therapeutic application.

Read Full Article HTML DOI: 10.29328/journal.jsctt.1001024 Cite this Article Read Full Article PDF

Keywords:

Adipose tissue; Cell viability; Cell yield; Feline; Mesenchymal; Omental; Stem cell; Regenerative medicine

References

  1. Al-Nbaheen M, Vishnubalaji R, Ali D, Bouslimi A, Al-Jassir F, et al. Human Stromal (Mesenchymal) Stem        Cells from Bone Marrow, Adipose Tissue and Skin Exhibit Differences in Molecular Phenotype and Differentiation Potential. Stem Cell Rev Rep. 2013; 9: 32–43. PubMed: https://pubmed.ncbi.nlm.nih.gov/22529014/
  2. Aliborzi G, Vahdati A, Mehrabani B, Hosseini SE. Isolation, characterization and growth kinetic comparison of bone marrow and adipose tissue mesenchymal stem cells of guinea pig. Inter J Stem Cells. 2016; 9: 115-123. PubMed: https://pubmed.ncbi.nlm.nih.gov/27426093/
  3. Archana M, Basha SH, Kannan TA, Mangalagowri A, William BJ, et al. Differentiation potential of ovine bone marrow derived mesenchymal stem cells. Int J Adv Res. 2015; 3: 1554-1557.
  4. Snedecor GM, Cochran WG. In: Statistical Method.8th edn; Oxford and IBN Publishing Company, New Delhi, India. 1994.
  5. Eca LP, Raalho RB, Oliveira IS, Gomes PO, Pontes P, et al. Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbit. Acta Cir Bras. 2009; 24: 400-404. PubMed: https://pubmed.ncbi.nlm.nih.gov/19851694/
  6. Scheubel RJ, Zorn H, Silber RE, Kuss O, Morawietz H, et al. Age-dependent depression in circulating endothelial progenitor cells in patients undergoing coronary artery bypass grafting. J Am Coll Cardiol. 2003; 42: 2073-2080. PubMed: https://pubmed.ncbi.nlm.nih.gov/14680729/
  7. Tokalov SV, Gruner S, Schindler S, Wolf G, Baumann M, et al. Age related changes in the frequency of mesenchymal stem cells in bone marrow of rats. Stem Cells Dev.2003; 16: 439-446. PubMed: https://pubmed.ncbi.nlm.nih.gov/17610374/
  8. Choudhery MS, Badowski M, Muise A, Dierce JP, Harris DT. Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation. J Transl Med. 2014; 12: 1-144. PubMed: https://pubmed.ncbi.nlm.nih.gov/24397850/
  9. Luna ACL, Madeira MEP, Conceicao TO, Moreira JALC, Laiso RAN et al. Characterization of adipose-derived stem cells of anatomical region from mice. Bio Med Central Res Notes. 2014; 7: 1-12. PubMed: https://pubmed.ncbi.nlm.nih.gov/25138545/
  10. Chen HT, Lee MJ, Chen CH, Chuang SC, Chang LF, et al. Proliferation and differentiation potential of human adipose-derived mesenchymal stem cells isolated from elderly patients with osteoporotic fractures. J Cell Mol Med. 2012; 16: 582-593. PubMed: https://pubmed.ncbi.nlm.nih.gov/21545685/
  11. Wu W, Niklason L, Steinbacher DM. The effect of age on human adipose-derived stem cells. Plast Reconstr Surg. 2013; 131: 27-37. PubMed: https://pubmed.ncbi.nlm.nih.gov/22965240/
  12. Kakudo N, Naoki M, Takeshi O, Kenji K. Potential of adipose-derived stem cells for regeneration  medicine; clinical application and usefulness of fat grafting. Stem Cell Ther. 2014; 4: 1-3.

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