Published: 31 July, 2017 | Volume 1 - Issue 1 | Pages: 028-051
7: A grouped bar graph of the mRNA expression of the osteogenic markers, a) ALP, b) osterix, at days 3, 7, 14 and 25. Error bars indicate standard deviation and connected treatments are significantly different. The amount of target normalized to housekeeping gene, GAPDH, and hASC values obtained prior to differentiation assays at day 0 were considered as the reference. NC: HG-DMEM/F12 supplemented with 1 mM sodium pyruvate, 2 mM L-glutamine, 5 μg/ml Gentamicin, 1% insulin-transferrin-selenium, 50 μM L-proline, and 1% penicillin-streptomycin; Dex: NC+100 nM dexamethasone; TGF: NC+10 ng/ml TGF-β3; Asc: NC+50 μg/ml L-ascorbic acid; DT: NC+100 nM Dex+10 ng/ml TGF-β3; DTA: NC +10 nM Dex+50 μg/ml Asc+10 ng/ml TGF-β3.