Published: 31 July, 2017 | Volume 1 - Issue 1 | Pages: 028-051
8: Bar graph representing the elastic modulus for each treatment group at day 3, 7 and 25 as indicated. Error bars indicate standard error of mean. Error bars indicate standard deviation and connected treatments are significantly different. The amount of target normalized to housekeeping gene, GAPDH, and hASC values obtained prior to differentiation assays at day 0 were considered as the reference. NC: HG-DMEM/F12 supplemented with 1 mM sodium pyruvate, 2 mM L-glutamine, 5 μg/ml Gentamicin, 1% insulin-transferrin-selenium, 50 μM L-proline, and 1% penicillin-streptomycin; Dex: NC+100nM dexamethasone; TGF: NC+10 ng/ml TGF-β3; Asc: NC+50 μg/ml L-ascorbic acid; DT: NC+100 nM Dex+10 ng/ml TGF-β3; DTA: NC+100 nM Dex+50 μg/ml Asc+10 ng/ml TGF-β3.