Figure 5

Enhancing adipose stem cell chondrogenesis: A study on the roles of dexamethasone, transforming growth factor β3 and ascorbate supplements and their combination

Bernard J Van Wie*, Arshan Nazempour##, Chrystal R Quisenberry## and Nehal I Abu-Lail

Published: 31 July, 2017 | Volume 1 - Issue 1 | Pages: 028-051

5: A grouped bar graph of the mRNA expression of the angiogenic marker, VEGF-A, at days 3, 7, 14 and 25. Error bars indicate standard deviation and connected treatments are significantly different. The amount of target normalized to housekeeping gene, GAPDH, and hASC values obtained prior to differentiation assays at day 0 were considered as the reference. NC: HG-DMEM/F12 supplemented with mM sodium pyruvate, 2 mM L-glutamine, 5 μg/ml Gentamicin, 1% insulin-transferrin-selenium, 50 μM L-proline, and 1% penicillin-streptomycin; Dex: NC+100 nM dexamethasone; TGF: NC+10 ng/ml TGF-β3; Asc: NC+50 μg/ml L-ascorbic acid; DT: NC+100 nM Dex+10 ng/ml TGF-β3; DTA: NC+100 nM Dex+50 μg/ml Asc+10 ng/ml TGF-β3.

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Figure 5:

5: A grouped bar graph of the mRNA expression of the angiogenic marker, VEGF-A, at days 3, 7, 14 and 25. Error bars indicate standard deviation and connected treatments are significantly different. The amount of target normalized to housekeeping gene, GAPDH, and hASC values obtained prior to differentiation assays at day 0 were considered as the reference. NC: HG-DMEM/F12 supplemented with mM sodium pyruvate, 2 mM L-glutamine, 5 μg/ml Gentamicin, 1% insulin-transferrin-selenium, 50 μM L-proline, and 1% penicillin-streptomycin; Dex: NC+100 nM dexamethasone; TGF: NC+10 ng/ml TGF-β3; Asc: NC+50 μg/ml L-ascorbic acid; DT: NC+100 nM Dex+10 ng/ml TGF-β3; DTA: NC+100 nM Dex+50 μg/ml Asc+10 ng/ml TGF-β3.

Read Full Article HTML DOI: 10.29328/journal.jsctt.1001004 Cite this Article Read Full Article PDF

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